Detection, quantification and visualisation of protein interactions for hit profiling by high conten

Alongside established drug target classes, innovative approaches are addressing previously undruggable target classes such as protein–protein interactions. The new Duolink® in situ kits are an excellent tool to visualize and quantify protein interactions in fixed cells and tissues samples – not only in a single experiment but also in high-throughput screening applications. The microtiter plate format allows for high-throughput screening of drug compounds affecting endogenous protein modifications and protein-protein interactions. Duolink®, which is based on the in situ PLA® (Proximity Ligation Assay) technology, is a highly sensitive and selective way of detecting proteins and enables the use of unmodified primary cells. Compound Profiling using PLA® can be used as part of the hit-to-lead workflow and the key advantage is the ability to provide evidence for target engagement of the compound.

This webinar will provide an overview of how the PLA® technology can be used on primary cells, stem cells, differentiated cells and cancer cell lines in a high-throughput screening format and how PLA® analysis can be performed in parallel to cell cycle, and compartment specific assessments.