Guanosine5'-(tetrahydrogen triph) (GTP-Na2)

Guanosine 5'-(tetrahydrogen triphosphate) disodium salt (GTP-Na₂) is a nucleotide derivative commonly used in molecular biology research and biochemistry. It is structurally similar to ATP (adenosine triphosphate) and functions as an energy source and as a substrate for various enzymatic reactions in living organisms.

Here's a brief introduction to GTP-Na₂:

Structure: GTP-Na₂ consists of a guanosine molecule, which is composed of a purine base (guanine) linked to a ribose sugar, with a phosphate group attached to the 5' carbon of the ribose. In GTP-Na₂, three additional phosphate groups are attached to the ribose, forming a triphosphate moiety.

Function: GTP-Na₂ plays a crucial role in cellular processes such as protein synthesis, signal transduction, and energy transfer. It serves as a high-energy molecule that provides the energy required for various enzymatic reactions, including GTP-binding proteins (G proteins) involved in signal transduction pathways.

Applications:

1. Protein synthesis: GTP is essential in translation, where it serves as an energy source for the elongation phase of protein synthesis. It participates in the binding of aminoacyl-tRNA to the ribosome, facilitating the addition of amino acids to the growing polypeptide chain.

2. Signal transduction: GTP is a key regulator of G protein-coupled receptor (GPCR) signaling. G proteins, upon activation by a GPCR, exchange their bound GDP (guanosine diphosphate) for GTP, leading to downstream signaling cascades.

3. Enzyme cofactor: GTP acts as a cofactor for various enzymes, including GTPases, which regulate processes such as cell division, membrane trafficking, and cytoskeletal rearrangement.

4. Research tool: GTP-Na₂ is widely used in biochemical and molecular biology experiments as a substrate, cofactor, or energy source. It is used in in vitro assays to study enzymatic activities, protein-protein interactions, and GTP-binding protein functions.

It's important to note that GTP-Na₂ is typically used in research settings and not for therapeutic or diagnostic purposes. Its stability, availability, and reliable enzymatic properties make it a valuable tool for studying cellular processes and molecular mechanisms in biological systems.