LNA-abasic phosphoramidite

LNA-abasic phosphoramidite is a special reagent used for nucleic acid synthesis. The following are its detailed information:

Structural features: It combines the structural characteristics of locked nucleic acid (LNA) and base-free (abasic). The LNA part locks the ribose in the C3' -endoform conformation by forming a methylene bridge between the 2' -oxygen atom and the 4' -carbon atom of the ribose. This structural restriction increases the rigidity and stability of nucleic acid analogues 2. The baseless part is the absence of the base part in traditional nucleic acids. Usually, a site that can be further modified or treated is introduced during the synthesis process to simulate the situation of base absence in nucleic acids or to serve as an active site for subsequent chemical reactions.

Application and function: It is mainly used in the field of nucleic acid chemical synthesis, especially when preparing oligonucleotides with special functions or structures, it is very useful. For example, when studying the relationship between the structure and function of nucleic acids, by introducing the LNA-abasic site, the physicochemical properties and biological activities of oligonucleotides can be changed, thereby deeply exploring the folding, hybridization and interaction with proteins of nucleic acids, etc. In the research and development of antisense oligonucleotides (ASOs) and aptamers, LNA-abasic phosphoramidite also has important applications. It can improve the stability, affinity and specificity of oligonucleotides and contribute to the development of more effective gene drugs and biosensors.

Synthesis method: It is generally prepared through multi-step organic synthesis reactions. Firstly, using the appropriate ribose or deoxyribose as the starting material, the LNA structural unit is constructed through a series of protecting group introductions and chemical reactions. Then, the synthesis or introduction of the base-free part is carried out at a specific position, and the phosphoramididation reaction is conducted to obtain LNA-Abasic phosphoramidite. The specific synthetic routes may vary among different research groups or manufacturers, but generally they all follow similar strategies and reaction steps.

Properties and stability: LNA-abasic phosphoramidite is usually a white to pale yellow solid or oily substance. During storage and use, it is necessary to avoid contact with water, acids, alkalis and other substances, as these substances may cause the protective group to fall off or the molecules to degrade. It is generally recommended to store it dry at -20 ℃ or lower to ensure its stability and activity. Under appropriate storage conditions, this compound can maintain its chemical properties and reactivity for a certain period of time. However, over time, it may gradually degrade, so its quality and purity need to be checked regularly.

Usage method: In nucleic acid synthesis, LNA-abasic phosphoramidite is usually used together with other conventional nucleic acid synthesis reagents for the synthesis of oligonucleotides through an automated nucleic acid synthesizer. During the synthesis process, it is necessary to select appropriate synthesis conditions, such as reaction time, temperature, reagent concentration, etc., based on specific experimental designs and requirements. Due to the introduction of the LNA structure, it may be necessary to appropriately extend the coupling reaction time during the synthesis process to ensure that the LNA-ABasic monomer can effectively attach to the oligonucleotide chain being synthesized 10. After the synthesis is completed, post-processing steps such as deprotection and purification of the product are still required to obtain high-quality oligonucleotides containing LNA-abasic sites.