C16 linker phosphoramidite

C16 linker phosphoramidite

Packaging specifications :1g/2g/3g/4g/5g/10g

Colorless oil

Structural features: It is usually composed of a 16-carbon long-chain alkyl group as the linker, with one end connected to a phosphoramidite group and the other end possibly connected to a protective group or other specific functional groups. The phosphoramidite group is the key part that exerts reactive activity in the synthesis of oligonucleotides and can undergo connection reactions with other nucleotide monomers or already synthesized oligonucleotide chains. The long-chain alkyl group of 16 carbon endows this reagent with certain hydrophobicity and steric hindrance properties, which can be used to introduce specific spacer or ligation functions in oligonucleotide synthesis.

Function and application: Mainly used for the modification and synthesis of oligonucleotides. Various functional groups or molecules can be attached to specific positions of oligonucleotides. For example, in the synthesis of antisense oligonucleotides, nucleic acid aptamers, etc., C16 linker phosphoramidite can be used to attach functional groups such as fluorescent dyes, biotin, and lipids to oligonucleotides, thereby endows oligonucleotides with specific biological functions or facilitating detection and separation. In addition, its long-chain structure can also serve as a spacer, reducing the steric hindrance between the modifying group and the oligonucleotide chain, improving the spatial conformation of the oligonucleotide, and facilitating its binding to the target molecule.

Synthesis method: Generally, it is prepared by chemically reacting a compound containing a 16-carbon long chain with phosphoramidite reagent. Firstly, appropriate functionalization of long-chain compounds is required to introduce active sites that can react with phosphoramidite groups. Then, under specific reaction conditions, a condensation reaction occurs between the two to form C16 linker phosphoramidite. During the synthesis process, protecting groups are usually needed to safeguard some sensitive functional groups to ensure the selectivity of the reaction and the purity of the product.

Properties and stability: Usually solid, sensitive to water and air, and prone to hydrolysis and oxidation reactions. Therefore, during storage and use, it is necessary to keep it dry and in an inert atmosphere. Generally, it is recommended to store it at -20 ℃ or lower to extend its shelf life and maintain its reactivity.

Relevant parameters: Products provided by different suppliers may have some differences, but generally speaking, their purity is usually required to be ≥98%. Regarding the specific molecular formula and molecular weight, they may vary depending on the specific groups and structures connected. For example, if it is 5' -amino-C16-MMT-CE Phosphoramidite(where MMT is dimethoxy-triphenylmethyl and CE is cyanoethyl), its molecular formula might be C42H58N3O4P and the molecular weight is approximately 709.9.