TNA-T phosphoramidite

TNA-C phosphoramidite

White powder, readily soluble in solvents such as dichloromethane, acetonitrile, and tetrahydrofuran, insoluble in water. Used for oligonucleic acid synthesis

TNA-C phosphoramidite is a phosphoramidite monomer used for nucleic acid synthesis. The following is its detailed information:

Structural features :TNA stands for threose nucleic acid, and the "C" in TNA-C phosphoramidite represents Cytosine. Its structure is based on stachyose as the sugar base skeleton, which is connected to the cytosine base through glycosidic bonds, and at specific positions of the sugar group, there is a phosphamide group that can be used in nucleic acid synthesis reactions. Unlike ribose or deoxyribose in natural nucleic acids, the structure of stachyose endows TNA with unique physical and chemical properties.

Function and application: Mainly used for the chemical synthesis of threefold nucleic acid (TNA). In the nucleic acid synthesizer, TNA-C phosphoramidite, as a monomer, is gradually linked with other TNA monomers or modified monomers through a series of chemical reactions to form TNA chains with specific sequences. TNA is of great significance in life science research. For example, as a potential genetic material analogue, it is used to explore the mysteries of the origin and evolution of life. It can also be used to prepare nucleic acid molecules with special functions, such as TNA enzymes with catalytic activity, etc.

Synthesis method: It is usually prepared through multiple organic synthesis reactions using stachyose and cytosine as starting materials. Firstly, an appropriate protecting group is introduced into stachyose to protect its active groups such as hydroxyl groups. Then, a glycosylation reaction is carried out with cytosine to form a TNA-C intermediate with a protecting group. Then, the phosphoramididation reaction was carried out on the intermediate to introduce the phosphoramididation group, and through steps such as deprotection, TNA-C phosphoramidite was obtained.

Properties and stability: Generally a white to off-white powder. This compound is relatively sensitive to water, acids, bases, etc. During storage and use, it should be avoided to come into contact with these substances to prevent hydrolysis, deprotection and other reactions that may cause damage to the molecular structure. It is usually recommended to store it dry at -20 ℃ or lower to maintain its stability and reactivity.

Relevant parameters :CAS number 325683-96-3, molecular formula C45H50N5O8P, molecular weight 819.896, purity ≥98%1.